Fig 1: RT induced a contradictory effect on DC function determined by TGF-β signaling. (A) The secretion levels of total TGF-β in the supernatant of irradiated U14 cells; N= 3, *p < 0.05. (B) The secretion levels of free active TGF-β in the supernatant of irradiated U14 cells; N= 3-5, *p < 0.05. (C) The supernatant of irradiated U14 cells was collected 24 h after radiation exposure. DCs were co-incubated with the supernatant for 24 h and the DC phenotype was analyzed by flow cytometry. (D) The supernatant of irradiated U14 cells was cocultured with DCs pretreated with TGF-β receptor antagonist (TGF-βi: galunisertib 50 μM), and the DC phenotype was analyzed. The expression of CD40, CD80, CD86, MHCI, and MHCII in CD11c+ cells was detected with flow cytometry. P., positive percentage; M., mean fluorescence intensity. (E) Bar graphs and statistical analysis of the FACS results; N= 3-5, *p < 0.05. All above data are representative results from two or three independent experiments. (F) Correlation analysis of TGFB1 expression and the expression of marker genes of infiltrating DCs in cervical cancer according to the TIMER database. (G) The level of TGFB1 expression in different tumor types according to the TCGA database in TIMER (CESC: cervical squamous cell carcinoma).